Sir John Lyttelton (1561–1601) was a Member of Parliament for Worcestershire during the reign of Queen Elizabeth I.
He was the eldest son of Gilbert Lyttelton and married Meriel, daughter of Sir Thomas Bromley, Lord Chancellor of England. They had three sons and eight daughters.
He was elected a knight of the shire for Worcestershire in 1584, 1586 and 1597.
He was concerned in the rebellion of Robert Devereux, 2nd Earl of Essex in 1599 and was in consequence tried for high treason, but died in prison in July 1601. In consequence, his estates (in Frankley, Halesowen, Hagley and Upper Arley) were forfeited to the Crown, but were restored to his widow Merriel on the accession of James I. She survived him 28 years and cleared the estates of debt, bringing up her children as Anglicans. The eldest became Sir Thomas Lyttelton, 1st Baronet.